A simple, precise, accurate and reproducible spectrophotometric method has been developed for Simultaneous estimation of Olmesartan medoxomil (OLM), Amlodipine besylate (AML) and Hydrochlorothiazide (HCTZ) by employing first order derivative zero crossing method in Methanol and Distilled water (70:30). The first order derivative absorption at 237.51 nm (ZCP of OLM and AML) was used for quantification of HCTZ, 316.96 nm (ZCP of OLM and HCTZ) for quantification of AML and 296.03 nm (ZCP of AML and HCTZ) for quantification of OLM. The linearity was established over the concentration range of 4-20 μg/ml, 4-20 μg/ml and 3-15 μg/ml for OLM, AML and HCTZ with correlation coefficient (r2) of 0.99904, 0.99873 & 0.99980 respectively. The mean % recoveries were found to be in the range of 98.31%–98.5%, 100.75%–101.5% and 99.70%–99.86% for OLM, AML and HCTZ respectively. Inter-day and intra-day studies showed repeatability of the method. The method is successfully applied to pharmaceutical formulation, with no interference from excipients as indicated by the recovery study. All validation parameters were within the acceptable range. The proposed method has been validated as per ICH guideline and successfully applied to the simultaneous estimation of OLM, AML, & HCTZ in their combined Tablet dosage form. The proposed method is recommended for routine analysis since it is rapid, simple, accurate and also sensitive and specific.
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